The Development of a Pulse-Spread Technique to Analyze the Directionality of Neurofilament Transport in Axons

Senior Honors Thesis 2 Acknowledgements I would like to thank Dr. Brown for giving me the opportunity to conduct research in his lab, awarding me with financial support, and providing with me his time, invaluable guidance, and encouragement. I would also like to thank Paula Monsma for her time and patience in teaching me instrumental lab techniques. Additionally, I would like to thank all of the members of the Brown Lab for welcoming me, creating a wonderful atmosphere to work in, and teaching me how to think like a scientist. Finally, I would like to thank my friends and family for all of their support throughout my undergraduate career. Amrita Mukhopadhyay did all of the imaging, analysis, and figure preparation for this thesis. She also did most of the transfection and staining prior to imaging. During the summer, Amrita established some cell cultures, but during the academic year, rat DRG cultures were established by Paula Monsma, a research assistant in the lab. Paula also conducted some of the transfection, staining, and DNA preparation protocols.